human uterine smooth muscle cells Search Results


primary human uterine smooth muscle cells  (ATCC)
94
ATCC primary human uterine smooth muscle cells
Primary Human Uterine Smooth Muscle Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary human uterine smooth muscle cells/product/ATCC
Average 94 stars, based on 1 article reviews
primary human uterine smooth muscle cells - by Bioz Stars, 2026-06
94/100 stars
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cd31 pregnant human uterine smooth muscle cells  (PromoCell)
94
PromoCell cd31 pregnant human uterine smooth muscle cells
Figure 3. Piezo1-meidiated Ca2+ influx in <t>CD31+</t> and CD31−human myometrial cells An intracellular calcium flux assay determined Piezo1 activity in phMEC (CD31+) and phUSMC (CD31−) cells. phMEC, phUSMC and HEK293 Piezo1KO cells were pre-treated with the Ca2+ indicator Calbryte (ex/em 493/515 nm) followed by exposure to Yoda1 (0.3 or 3 μM) ± the Piezo1 antagonist Dooku1 (10 μM) and the change in fluorescence (Cai2+) was measured. A, phMECs treated with 3 μM Yoda1 exhibited 4.09-fold increase in Ca2+ uptake (Cai2+) over 0.3 μM treated cells (P < 0.0001) which decreased by 35.74% when co-treated with Dooku1 (P = 0.0327) at 60 min. B, phUSMC experienced a 2.64-fold increase in fluorescence when challenged with 3 μM Yoda1 (P < 0.0001), with a respective decrease of 22.49% when co-treated with Dooku1 (P = 0.0326). C, Piezo1KO fluorescence did not vary significantly at any dose of Yoda1 or Yoda1 + Dooku1 relative to baseline (Kruskal–Wallis one-way ANOVA, P = 0.2622). D, left, ×10 bright field images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Right, Calbryte-induced fluorescence after 3 μM Yoda1 stimulation. ×20 fluorescence images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Data presented as ±SD.
Cd31 Pregnant Human Uterine Smooth Muscle Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd31 pregnant human uterine smooth muscle cells/product/PromoCell
Average 94 stars, based on 1 article reviews
cd31 pregnant human uterine smooth muscle cells - by Bioz Stars, 2026-06
94/100 stars
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utsmc - human uterine smooth muscle cells  (Lonza)
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UtSMC, Uterine Smooth Muscle Cells
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human uterine smooth muscle cells  (AcceGen Biotechnology)
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Smooth muscle tissue is found in the tunica media layer of large and small blood vessels and in the walls of hollow organs like the bladder and the uterus. Smooth Muscle Cells (SMC) possess all
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human uterine smooth muscle cells (hutsmc), cell pellet in rnalater  (PromoCell)
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Cell pellet of HUtSMC from the myometrium in RNAlater® for subsequent RNA, DNA or protein analysis. Cell pellet consisting of 1 million cells dissolved in 200 µl RNAlater® for subsequent RNA, DNA or protein analysis.
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human uterine smooth muscle cell complete medium  (Elabscience Biotechnology)
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Image Search Results


Figure 3. Piezo1-meidiated Ca2+ influx in CD31+ and CD31−human myometrial cells An intracellular calcium flux assay determined Piezo1 activity in phMEC (CD31+) and phUSMC (CD31−) cells. phMEC, phUSMC and HEK293 Piezo1KO cells were pre-treated with the Ca2+ indicator Calbryte (ex/em 493/515 nm) followed by exposure to Yoda1 (0.3 or 3 μM) ± the Piezo1 antagonist Dooku1 (10 μM) and the change in fluorescence (Cai2+) was measured. A, phMECs treated with 3 μM Yoda1 exhibited 4.09-fold increase in Ca2+ uptake (Cai2+) over 0.3 μM treated cells (P < 0.0001) which decreased by 35.74% when co-treated with Dooku1 (P = 0.0327) at 60 min. B, phUSMC experienced a 2.64-fold increase in fluorescence when challenged with 3 μM Yoda1 (P < 0.0001), with a respective decrease of 22.49% when co-treated with Dooku1 (P = 0.0326). C, Piezo1KO fluorescence did not vary significantly at any dose of Yoda1 or Yoda1 + Dooku1 relative to baseline (Kruskal–Wallis one-way ANOVA, P = 0.2622). D, left, ×10 bright field images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Right, Calbryte-induced fluorescence after 3 μM Yoda1 stimulation. ×20 fluorescence images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Data presented as ±SD.

Journal: The Journal of Physiology

Article Title: Novel identification and modulation of the mechanosensitive Piezo1 channel in human myometrium

doi: 10.1113/jp283299

Figure Lengend Snippet: Figure 3. Piezo1-meidiated Ca2+ influx in CD31+ and CD31−human myometrial cells An intracellular calcium flux assay determined Piezo1 activity in phMEC (CD31+) and phUSMC (CD31−) cells. phMEC, phUSMC and HEK293 Piezo1KO cells were pre-treated with the Ca2+ indicator Calbryte (ex/em 493/515 nm) followed by exposure to Yoda1 (0.3 or 3 μM) ± the Piezo1 antagonist Dooku1 (10 μM) and the change in fluorescence (Cai2+) was measured. A, phMECs treated with 3 μM Yoda1 exhibited 4.09-fold increase in Ca2+ uptake (Cai2+) over 0.3 μM treated cells (P < 0.0001) which decreased by 35.74% when co-treated with Dooku1 (P = 0.0327) at 60 min. B, phUSMC experienced a 2.64-fold increase in fluorescence when challenged with 3 μM Yoda1 (P < 0.0001), with a respective decrease of 22.49% when co-treated with Dooku1 (P = 0.0326). C, Piezo1KO fluorescence did not vary significantly at any dose of Yoda1 or Yoda1 + Dooku1 relative to baseline (Kruskal–Wallis one-way ANOVA, P = 0.2622). D, left, ×10 bright field images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Right, Calbryte-induced fluorescence after 3 μM Yoda1 stimulation. ×20 fluorescence images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Data presented as ±SD.

Article Snippet: Cells captured by the beads were deemed CD31+ pregnant human myometrial endothelial cells (phMEC) and CD31− pregnant human uterine smooth muscle cells (phUSMC). phMECs cultured in endothelial basal medium 2 (C-22 011, PromoCell, Heidelberg, Germany) containing 10% fetal bovine serum (FBS) and 1% penicillin, while the phUSMC cells were cultured in DMEM with 50 U/ml streptomycin, 50 μg/ml penicillin, and 10% FBS and supplemented with oestrogen (15 ng/ml) and progesterone (200 ng/ml).

Techniques: Calcium Flux Assay, Activity Assay, Fluorescence